Protein_Domain
ZipE34

Part:BBa_J18923

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-26)

E34(I) strong heterodimerizing leucine zipper

Engineered Leu zipper based on VBP B-ZIP domain that forms strong parallel heterodimers with R34.

Interaction with R34: 

  * Kd = 6.1 nM

(CRG-internal ID: rg0109)


References

  • Acharya et al. (2002) A heterodimerizing leucine zipper coiled coil system for examining the specificity of a position interactions: amino acids I, V, L, N, A, and K. PMID: 12450375
  • Bashor et al (2008) Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics. PMID: 18339942


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/binding
Parameters
None